Application areas are in structural analysis, protein interactions, signal transduction, life sciences, single molecule detection, biochip development, food processing, biophysical studies and colloid Chemistry.
Information obtained by light microscopy
Localisation. Specific staining or fluorescent labels allow temporal and spatial localisation of your (bio)molecule of interest. A range of fluorescent probes can be combined for simultaneous multicolor, multidimensional image acquisition.
Morphology and 3D reconstruction. The shape, 3D volume and interaction of molecules, organelles or tissues can be derived from optical sections from confocal microscopy.
Analysing (sub)micron dynamics. Rapid image acquisition of fluorescent probes allows imaging (relative) dynamics that can be analysed in kymographs. FRAP studies give insight in fluorescent displacements and allow quantification of protein dynamics.
Polarization microscopy. Biopolymers are made out of molecules arranged in a specific order and as a consequence have a birefringent characteristic. Protein crystals, collagen, cell wall polymers, microtubules or actin filament bundles are examples. Crystallographic and polymer orientation information can be obtained.