• Supplier: Thermo Scientific Instruments Specifications: Q Exactive Focus MS attached to a Thermo Vanquish Liquid Chromatography system. The Thermo Scientific Q Exactive Focus benchtop LC-MS/MS combines high-performance quadrupole precursor selection with very high resolution, accurate-mass Orbitrap FTMS detection to deliver high performance and tremendous versatility. All features are essential for the envisaged applications and specifications are greatly advanced compared to the existing old instruments. The key features are:
• Resolving power up to 70,000 (FWHM) at 200 m/z
• General mass range 100 – 1,200 Da up to 500 – 6,000 Da
• Mass accuracy < 1 ppm
• Maximum scan speed 12Hz
• Intra-scan dynamic range > 5000:1
• Quadrupole mass filter
• C-trap precursor ion trapping
• High-energy collisional dissociation (HCD)
• Spectral multiplexing for enhanced duty cycle
• S-Lens ion source for increased sensitivity
• HESI ion Source
• Vanquish UPLC separation
• Diode Array detection is possible
Software
Using dedicated software (Compound Discoverer 2.0) for untargeted data analyses, large series of samples can be compared for differences and similarities in the relative abundance of all small molecules detected, both known and yet unknown compounds.
This software package can identify compounds using the accurate mass MS, MS/MS and the isotope pattern in the search in several databases like mzCloud™, Chemspider, KEGG. Compound Discoverer 2.0 can predict possible metabolites from these identified compounds and uses this information to identify more unknown compounds in the samples. For quantification of targeted compounds a special software package, TraceFinder 4.0, is available.