This confocal microscope (Microtime 200 from Picoquant) can analyze a multitude of parameters down to the single molecule level using methods such as Fluorescence Lifetime Imaging (FLIM), Förster Resonance Energy Transfer (FRET), Fluorescence Correlation Spectroscopy (FCS), Fluorescence Lifetime Measurements, Fluorescence Anisotropy and Fluorescence Intensity Time Traces.
The microscope is paired with a supercontinuum laser for 405-630nm with wavelength selection module (Leukos) and a picosecond laser source (640 nm) as a second laser source. Furthermore, it has a Time-Correlated-Single Photon Counting (TCSPC) module with 1 ps resolution, two Single-Photon Avalanche Diode (SPAD) detectors and a Hybrid detector for fast FLIM.
The Microtime confocal is connected to the JPK Nanotracker 2 optical tweezers platform based on research-grade inverted optical microscopes and designed for sensitive manipulation, force and tracking experiments. With the NanoTracker 2, you can trap and track particles from several µm down to 30nm with the ability to control, manipulate and observe samples in real time with nanometer precision and femtoNewton resolution.