Description
With Oroboros Oxygraph O2k you are able to measure real-time oxygen (O2) consumption/flux in a variety of samples such as living cells, permeabilized tissues and cells, and isolated mitochondria. The experimental data provides you detailed information about the bioenergetics and (specific) OXPHOS activity of your tissue of interest.
Technical Details
We offer three independent Oroboros Oxygraph O2k. It uses an polarographic O2-sensor (POS) to measure real-time the of oxygen concentration (µM) in the medium. In time, the oxygen concentration will decline as the biological sample uses oxygen to produce cellular energy. By using software, this data can be transformed to display real-time oxygen consumption (pmol.s-1.ml-1). It has full flexibility in the ability to inject many different compounds sequentially. Many standardized protocols are already available, but tailored made protocols are also possible. This allows you to fully investigate the bioenergetic and OXPHOS properties of your biological sample.
Applications
- Bioenergetic profiling of living cells, permeabilized cells and tissue, and isolated mitochondria.
- Analyzing the effect of interventions or disease on bioenergetic profiles of biological samples
Complementary Techniques
The Seahorse XFe96 analyzer; Analysis of mitochondrial (OXPHOS) proteins and genes (western blot, RT-qPCR); Analysis of mitochondrial morphology (imaging).
Link to supplier Oroboros