Description
DNA Size Selection for Next-Gen Sequencing with Pulsed-Field Size-Selection for fragments up to 50kb (range 100 bp – 50 kb).
Applications
DNA (or RNA) size selection and purification for broad range of Downstream Technologies including DNA sequencing, genotyping, ChIP-seq, Long-read sequencing (PacBio, Oxford Nanopore). Pre casted gel cassettes for DNA selections are available in different agarose concentrations to provide a full range of size selection options between 100bp up to 50kb. For smaller fragment sizes, internal standards may be used for higher accuracy and increased sample capacity (from 4 to 5 samples/cassette). Higher molecular weight fragments require a dedicated lane to run a calibration marker. To resolve very large DNA fragments, pulsed-field runs are applied.