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Proteomics Orbitrap Exploris

Description

Proteomics by mass spectrometry using a Orbitrap Exploris

 

Sensitive and accurate protein identification and quantitation is done by separating and measuring peptide digests with a nanoLC - electrospray linked Orbitrap Exploris 480 mass spectrometer. Proteins from highly diverse origin (e.g. viruses, micro-organisms, plants, mammals) can be characterized using this technique. Proteomics sample preparation protocols are present for identification as well as for quantitation proteomic research questions. The standard MS method used for quantitation is label-free quantitation but labelled samples (e.g. dimethyl labelling or TMT) can be measured as well. To facilitate optimal measurement of different sample complexities, different nLC gradient lengths can be chosen suitable for measuring simple (in 1/2 h gradient) to highly complex protein mixtures (> thousand(s) proteins / 2h gradient).

Next to our principal bottom-up peptide approach described above, large peptides (middle-down) and even complete proteins (top-down, for example IgG ) can be measured as well but will need some tweaking on the sample preparation level and on the instrument control.

Technical Details

Applications

  • Bottom-up, middle-down and top-down proteomics
  • Phospho-proteomics
  • Glyco-proteomics


Last edited by Daniella Stijnen on 2022-09-21

Our expert(s)
Sjef Boeren
Contact our expert(s)
Brand

Thermo Fisher Scientific

Type

Orbitrap Exploris 480

Organisation

Department of Agrotechnology and Food Sciences
Shared Research Facilities

Subdivision

Biochemistry



To explore the potential of nature to improve the quality of life.

This Shared Research Facilities equipment database offers researchers from universities, research institutes and companies, including start-ups, the opportunity to find research equipment with their relevant expert and to use it, generally on a pay-per-use basis. The equipment is available at different locations at Wageningen Campus or other (company) locations. If you want to share your own research devices or if you need more information, please contact us.