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Axioscope fluorescence microscope with cooling stage


The Zeiss Axio Scope is an upright light microscope which can be operated with transmitted and reflected light. It can be used for brightfield and fluorescence microscopy. The microscope can be equipped with a programmable temperature controlled stage, by which the temperature of the sample can be varied from -25 to 120°C.

This equipment is located at Surfix (Plus Ultra, Bronland 12, Wageningen Campus). 

Technical Details

Light sources

  • transmitted light: halogen (50 W);
  • reflected light: metal halide (75 W).

Reflector modules

  • fluorescence filter set 38 (exc 470/40 nm, em 525/50 nm);
  • fluorescence filter set 20 (exc 546/12 nm, em 575-640 nm);
  • brightfield reflected light.

Objectives: 5x 10x 20x

Ocular: PL 10x/23

Camera: Axiocam 503 monochromatic

Software: Zen 2.3 lite (blue edition)

Temperature controlled stage (PE120, Linkam)

  • temperature range: -25 – 120 oC (Peltier);
  • control and stability: 0.1 oC;
  • heating/cooling rate: 0.1 – 20 oC/min;
  • sample area 35x32 mm2, 5 mm aperture for transmitted light;
  • programmable (standalone controller).


The Zeiss Axio Scope is a general purpose upright light microscope for materials research applications.

Last edited by Oscar de Vos on 2022-02-01

Our expert(s)
Anke Trilling
Contact our expert(s)

Carl Zeiss BV


Axioscope A1


Surfix BV
Shared Research Facilities


Surfix BV

To explore the potential of nature to improve the quality of life.

This Shared Research Facilities equipment database offers researchers from universities, research institutes and companies, including start-ups, the opportunity to find research equipment with their relevant expert and to use it, generally on a pay-per-use basis. The equipment is available at different locations at Wageningen Campus or other (company) locations. If you want to share your own research devices or if you need more information, please contact us.